Home > Sandwich ELISA Kits > Mouse 90kDa Heat Shock Protein Alpha A1 (HSP90αA1) ELISA Kit

Product Name:

Mouse 90kDa Heat Shock Protein Alpha A1 (HSP90αA1) ELISA Kit

Cat.#:

JL33598

Regulatory Status:

RUO

Alternative:

90kDa heat shock protein alphaA1；HSP90AA1; EL52; HSP86; HSP89A;HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP-2; LAP2; HEL-S-65p; Heat shock protein 90kDa alpha; heat shock protein 90kDa alpha family class A member 1; heat shock p

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.37ng/mL

Specificity:

This kit recognizes Mouse HSP90αA1 in samples. No significant cross-reactivity or interference between Mouse HSP90αA1 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| HSP90αA1

Heat shock protein HSP 90-alpha is a protein that encoded by the HSP90AA1 gene.The gene, HSP90AA1, encodes the human stress-inducible 90-kDa heat shock protein alpha (Hsp90A). Complemented by the constitutively expressed paralog Hsp90B which shares over 85% amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse HSP90αA1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-107	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse HSP90αA1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-114
1 : 4	Average (%)	94	98	107